Probe compounds and method development for the analysis of ROS in PT
This project investigates the role of reactive oxygen species (ROS) in DNA damage during proton therapy, with a focus on enhancing effects through sensitizers. Due to their short lifespans and low, fluctuating concentrations, ROS are difficult to detect, especially in complex environments like nanoparticle suspensions, simulated brain fluid, or microgels. To address this, we are developing and validating ROS probe compounds and analytical methods compatible with these advanced matrices, enabling precise quantification of ROS formation and decomposition under irradiation.